It is closing time at the Krusty Krab, and Mr. Krabs tells SpongeBob that he is going to play a card game against Plankton. SpongeBob is shocked at this. Mr. Krabs explains that Plankton is the worst card player in Bikini Bottom, and he is been winning every week for the last 15 years. In the morning, he tells SpongeBob that he lost, and Mr. Krabs bets SpongeBob's contract. SpongeBob is then taken to the Chum Bucket and forced to work for Plankton, who commands him to make a Krabby Patty, threatening to remove SpongeBob's brain and put it in a robot if he refuses to do what he is told. However, SpongeBob is unable to work in the Chum Bucket's cold, laboratory-like kitchen and Karen suggests Plankton to show SpongeBob compassion and understanding so that he will obey him. Plankton goes to great lengths to accommodate SpongeBob's working conditions, eventually converting the Chum Bucket kitchen into an exact replica of the Krusty Krab kitchen. However, SpongeBob is still not satisfied, and demands even more of Plankton, acting like a spoiled brat.
Eventually, Plankton takes out SpongeBob's brain and implants it in the robot as a punishment but the robot turns out to be a total failure. The robot refuses to make a Krabby Patty, so Plankton restores SpongeBob back to normal and gives him back to Mr. Krabs, saying that he cannot stand him and admitting that he cheated in the card game. Mr. Krabs welcomes him back and encourages SpongeBob to return to work. SpongeBob still does not want to make a Krabby Patty, but reveals that he was just kidding and quickly goes back to work.
There was a deleted scene where Plankton was taking out SpongeBob's brain before he puts SpongeBob's brain into the robot. 
The book Plankton reads to SpongeBob is also seen in "Grandma's Kisses." Even though the plots seem to be different. In this episode it was about Sea Elves. In Grandma's Kisses it was about a Sea Leprechaun.
It is possible that this book contains multiple stories.
Mr. Krabs' singing voice in this episode is similar to Louis Armstrong.
Plankton breaks the fourth wall when he says to the audience "I put the brain in the robot, you know..."
This episode was the subject of a clue on the episode of Jeopardy! that aired on October 10, 2005, during Back to School Week.
Things Plankton did to try to get SpongeBob to make a Krabby Patty:
Krusty Krab kitchen replica
Pushing him on a rolling seahorse
Reading him a book
Not hitting him
Rubbing his feet
Plankton put SpongeBob's brain into Robot SpongeBob's positronic head. If Plankton took out SpongeBob's brain and then put it back in after (he put it back into SpongeBob's head when Robot SpongeBob would not take commands), SpongeBob would have died when the brain was taken out, because once a brain is taken out, it cannot be put back into a head without the person resulting in death.
This is the second episode to have computerized picture-shaking effects. They occur whenever Plankton moves the grill. The first time to use these effects was "Neptune's Spatula."
The premise of Robot SpongeBob is similar to the plot of The Simpsons' Treehouse of Horror II segment featuring Homer's nightmare. Homer, a lazy worker, is kidnapped by Mr. Burns and Smithers, who put his brain into a robot. The robot ends up being lazy and refusing to work, just like Homer.
The PlayStation version of SuperSponge shows clips of this episode in the "introducing Mr. Krabs and Plankton" scene. It is unknown how the clips could have gotten in the game because SuperSponge was released about two months before this episode aired.
The scene where SpongeBob and Krabs singing as they missed each other was rarely shown in Indonesia. This was done due to time constraints as its airings were paired with ads.