She is a light green elderly fish with light blue hair that is pulled back into a bun with big pink lips. She wears a navy blue and white uniform, a whistle around her neck, golden glasses, and tall brown boots. She and her sisters share a similar appearance.
At some point in her life, she married Al Priss.
At another point, she founded T.U.O.O.F.A.T.T.A.F.A.D. with her sisters. She had banned Krabby Patties from being sold at the Krusty Krab with T.U.O.O.F.A.T.T.A.F.A.D. However she later unbanned them.
Role in series
"Banned in Bikini Bottom"
She wants Krabby Patties to be banned because they are "too fun and delicious." She has her husband close the Krusty Krab, and with the help of Plankton, destroys SpongeBob's house, where they are selling Krabby Patties illegally.
She then plans to arrest SpongeBob and Mr. Krabs for circumventing a law. However, when she sees her husband eating a Krabby Patty, she runs over to stop him, but in doing so, she trips over her untied shoelace and falls in front of her husband. The patty drops into her mouth; after she eats it, she realizes that Krabby Patties are not so bad after all.
Thus, she decides to unban Krabby Patties, pardon the Krusty Crew, and never close the Krusty Krab again.
Even though she calls herself Miss Gristlepuss, she should be called Mrs. Gristlepuss, because she is married.
In reality, Miss Gristlepuss has no power of closing down the Krusty Krab and banning Krabby Patties just for the reason of it being "fun and delicious," even if her husband is the chief of police. They must have a better reason, like it being poisonous would be a reason for it getting banned, but if the previous is true, and the Krusty Krab is still closed down, they can be arrested for false accusations.
In the end credits and TV guide description, Miss Gristlepuss is mistakenly called "Miss Priss."