Miss Gristlepuss is the leader of the United Organization of Fish Against Things That are Fun and Delicious (T.U.O.O.F.A.T.T.A.F.A.D.). She first appears in the episode "Banned in Bikini Bottom" as the main antagonist. Her husband is head of police.
She has light green skin and has light blue hair pulled back into a bun with big pink lips. She wears a navy blue and white uniform, a whistle around her neck, gold colored glasses, and tall brown boots. She and her sisters share a similar appearance.
At another point, (presumably after she married), she founded T.U.O.O.F.A.T.T.A.F.A.D. with her sisters. She had banned Krabby Patties from being sold from the Krabby with T.U.O.O.F.A.T.T.A.F.AD. then later unbanning.
She wanted Krabby Patties to be banned because they were 'too fun and delicious.' She had her husband close the Krusty Krab and with the help of Plankton, destroyed SpongeBob's home where they were selling Krabby Patties illegally. She then planned to arrest SpongeBob and Mr. Krabs. However, when she sees her husband eating a Krabby Patty, she runs over to stop him. But in doing so, she trips over her untied shoelace and falls in front of her husband. The patty drops into her mouth, and after she eats it, she realized that Krabby Patties are not so bad after all. Soon after, she decides to unban Krabby Patties, pardon the Krusty Crew and never close the Krusty Krab again.
T.U.O.O.F.A.T.T.A.F.A.D. is a group dedicated to banning foods it doesn't like, otherwise known as the food police. This may be based on the real-life food police group the Center for Science in the Public Interest, aka the CSPI.
Even though she calls herself Miss Gristlepuss, she should be called Mrs. Gristlepuss, because she is married.
In the end credits and TV guide description, Miss Gristlepuss is mistakenly called "Miss Priss."