She has light olive-green skin and has light blue hair pulled back into a bun with big pink lips. She wears a blue and white uniform, a whistle around her neck, gold colored glasses, and boots. She and her sisters share a similar appearance.
T.U.O.O.F.A.T.T.A.F.A.D. is a group dedicated to banning foods it doesn't like, otherwise known as the food police. This may be based on the real-life food police group the Center for Science in the Public Interest, aka the CSPI.
In the credits and TV Guide description, Miss Gristlepuss is mistakenly called Miss Priss.
Despite being married, Miss Gristlepuss is still called "Ms." and "Miss" instead of "Mrs."