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When everyone was stuck in the air vent. It was red and spied on SpongeBob's pineapple, Patrick's rock, Sandy's treedome, and apparently the room itself. When Squidward discovered this, he was angry at Mr. Krabs. Mr. Krabs fumbled for an excuse and said the room was built so he can make sure they floss after every meal. This room seems to be only accessible by going through the air vent, yet Mr. Krabs still knew about it. That means Mr. Krabs knew the air vent path well, and hid it from everyone.